Journal: International journal of molecular sciences

Article Title: Pentagalloyl Glucose (PGG) Exhibits Anti-Cancer Activity against Aggressive Prostate Cancer by Modulating the ROR1 Mediated AKT-GSK3β Pathway.

doi: 10.3390/ijms25137003

Figure Lengend Snippet: Figure 4. PGG inhibits ROR1 intracellular domain phosphorylation, ROR1 expression, down- stream oncogenic signaling, and anti-apoptotic signaling in PC3 cells. (A) Representative western blot of phospho-ROR1 (Tyr786) and ROR1 on PC3 cells treated with vehicle or 31.25 µM PGG for 24 h. (B) Quantification of immunoblot bands phospho-ROR1 (Tyr786) and ROR1, all nor- malized to GAPDH. Data are expressed as the mean +/−SEM from three independent western blots. (C) Representative western blot of phospho-AKT (ser473), phospho-GSK3β (ser9), AKT, and GSK3β on PC3 cells treated with vehicle or 31.25 µM PGG for 24 h. (D) Quantification of im- munoblot bands phospho-AKT (ser473), phospho-GSK3β (ser9), AKT, and GSK3β, all normalized to GAPDH. (E) Representative western blot of apoptotic markers XIAP, phospho-Bad (ser136), and Bad after treatment of PC3 cells with vehicle or 31.25 µM PGG for 24 h. (F) Quantification of immunoblot bands XIAP, phospho-Bad (ser136), and Bad, all normalized to GAPDH. Data are ex- pressed as the mean +/−SEM from three independent western blots. [N = 3. *** = p value < 0.001; * = p value < 0.05; ns = not significant].

Article Snippet: 2024, 25, 7003 18 of 24 XIAP (Cell signaling, USA, #14334S); Bad (Cell signaling, USA, #9239S); p-Bad-ser136 (Cell signaling, USA, #4366S); Snail (Cell signaling, USA, #3895S); Twist1 (Cell signaling, USA, #69366S); MMP-9 (Cell signaling, USA, #13667S); Cyclin A2 (Cell signaling, USA, #4656S); Cyclin D1 (Cell signaling, USA, #2978S); GAPDH (scbt, Santa Cruz, CA, USA, #C2819); HSP40 (Cell signaling, USA, #4868S); IRDye 800CW Goat anti-rabbit secondary (Licor, Lincoln, NE, USA, #926-32211); IRDye 800CW Goat anti-mouse secondary (Licor, USA, #926-32210); HRP conjugated Goat anti-mouse secondary (Biorad, Hercules, CA, USA, #1706516); HRP conjugated Goat anti-rabbit secondary (Cell signaling, USA, #7074S).

Techniques: Phospho-proteomics, Expressing, Western Blot